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Finally, blots were washed during 5 minutes three times with TTBS and detection was carried out using ECL Western blotting detection reagent (Amersham, GE HealthcareBio-Sciences AB, Uppsala, Sweden)

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Given that these pathways converge on the intersecting regulatory level of methylation potential-oxidative stress-inflammatory signaling, this section discusses their representative mechanisms and their effects on macrophage phenotypes together

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